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6 de ago. de 2020 · Quantitative measurements of biomolecule associations are central to biological understanding and are needed to build and test predictive and mechanistic models.
6 de ago. de 2020 · Full text. Figures and data. Peer review. Side by side. Abstract. Quantitative measurements of biomolecule associations are central to biological understanding and are needed to build and test predictive and mechanistic models.
5 de ene. de 2022 · Every reaction has characteristic values of affinity and specificity. We quantify affinity—the strength of binding—using ΔG, the free energy change between the bound and free (unbound) states of the partners.
16 de oct. de 2014 · Abstract. Protein-protein interactions (PPI) in nature are conveyed by a multitude of binding modes involving various surfaces, secondary structure elements and intermolecular interactions. This diversity results in PPI binding affinities that span more than nine orders of magnitude.
1 de jun. de 2017 · The data from these experimental techniques provide valuable information on important interactions which influence the affinity of protein–protein complexes, binding site residues at the interface and thermodynamic parameters for understanding the mechanism of protein–protein recognition at molecular level [ 1 ].
Molecular Basis of High Affinity Interactions. What differentiates high and low affinity binding at the molecular level? Do high affinity interactions have lots of intramolecular H-bonds, salt bridges, van der Waals interactions, or are hydrophobic interactions most important?
29 de nov. de 2021 · This observation indicates that binding affinity is a more effective predictor of substrate specificity than k cat /K m. Our findings have important biological and medicinal implications warranting additional considerations for enzyme substrate and inhibitor discovery.
