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Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass ...
Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals.
20 de jul. de 2017 · For single-channel recordings and whole-endolysosome recordings in voltage-clamp mode, it is possible to use a HEKA EPC10 (HEKA Instruments, cat. no. 895000) or an Axopatch 200B (Molecular...
1 de nov. de 2007 · Electrophysiological recording of single-channel currents is the most direct method available for obtaining detailed and precise information about the kinetic behavior of ion channels.
1. Introduction. The ability to record the real-time movement of a protein is quite enthralling; in effect, this is what we achieve when we record the activity of an individual ion channel.
11 de nov. de 2013 · Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its...
The present review describes methods used for single- channel recording and analysis, as applied to the calcium release channels in striated muscle, and details some of the unique contributions that single-channel recording and analysis have made to our current understanding of the release of Ca2+ from the internal stores of muscle. 6.
