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18 de dic. de 2020 · Gene knock-out (KO) is a critical method for identifying the functions of coding and non-coding genomic regions. Recent emergence of genome editing tools such as CRISPR/Cas9 can be used to...
Learn how to use CRISPR to create knockout cells or animals by introducing a double-strand break in the target gene. Find out how to optimize gRNA design, enhance specificity, and choose the best Cas protein for your experiment.
Learn how to generate and validate CRISPR-Cas9 knockout cell lines using guide RNA formats, transfection methods, and molecular techniques. Avoid common pitfalls and challenges in the CRISPR workflow with abcam's ready-made KO cell lines.
21 de dic. de 2017 · CRISPR/Cas9-based genome editing can easily generate knockout mouse models by disrupting the gene sequence, but its efficiency for creating models that require either insertion of exogenous...
Here, we present guidelines and tools to optimize CRISPR/Cas9 genome-targeting efficiency and specificity. The nature of the target locus, the design of the single guide RNA and the choice of the delivery method should all be carefully considered prior to a genome-editing experiment.
21 de ago. de 2021 · CRISPR/Cas-9 gene editing is adopted from acquired immunity in prokaryotes and consists of two elements: guide RNA used to locate (bind) the target DNA to be edited and Cas-9, a protein that essentially cuts the DNA at the location identified by guide RNA.
Science. 20 Jan 2023. Vol 379, Issue 6629. DOI: 10.1126/science.add8643. A decade of CRISPR. In the decade since the publication of CRISPR-Cas9 as a genome-editing technology, the CRISPR toolbox and its applications have profoundly changed basic and applied biological research.
