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13 de may. de 2024 · CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) y Cas9 (CRISPR-associated protein 9) son componentes clave de un sistema de defensa inmunitaria bacteriano que ha sido adaptado para la edición precisa y eficiente del ADN en diversos organismos, incluidos humanos, plantas y animales.
13 de may. de 2024 · Pero no fue hasta las décadas de 1990 y 2000 cuando los avances en biología molecular y genética permitieron modificar los órganos de animales para hacerlos compatibles con el cuerpo humano: una tarea que ahora se ha simplificado gracias a la creación de la técnica CRISPR-Cas9, también conocida como el "corta-pega" de la ...
30 de may. de 2024 · In this review, we summarized the genetic modification by CRISPR/Cas and CRISPR screening system, discussed key components for successful CRISPR screening, including Cas enzymes, guide RNA (gRNA) libraries, target cells or organs.
Hace 23 horas · Each embryo was injected with 2 nl of the RNP mixture at a Cas9 or PE2 protein to gRNA or pegRNA molar ratio of 1:2 (750 ng/µl of PE2 with 240 ng/µl of pegRNA, Figure 2; 500 ng/µl of Cas9 with 3 mixed pegRNAs of 80 ng/µl each, Figure 1—figure supplement 1, Figure 2—figure supplement 3) and immediately transferred to an incubator at 32 °C for PE2 and 28.5 °C for Cas9.
13 de may. de 2024 · In the present study, we identified a central role for genetic variation in genome editing by CRISPR–Cas9 by demonstrating that the presence of SNPs at key positions along the protospacer ...
29 de may. de 2024 · Comprehensive analyses of Cas9 proteins shed light on the evolution of the CRISPR–Cas9 system, and identify a pro-CRISPR accessory protein in bacteria that boosts CRISPR-mediated immunity...
29 de may. de 2024 · A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations. Aedes aegypti is the main vector of several major pathogens including dengue, Zika and ...
