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6 de ago. de 2020 · kequil = kon[P] + koff. (1) kon is the association rate constant, [P] is the concentration of protein, or the binding partner in excess, and koff is the dissociation rate constant ( Pollard, 2010 ). According to Equation 1, equilibration is the slowest at the lowest protein concentrations.
23 de may. de 2022 · We show that ProBound quantifies transcription factor (TF) behavior with models that predict binding affinity over a range exceeding that of previous resources; captures the impact of DNA...
To address this issue, we introduce PPI-Affinity, a tool that leverages support vector machine (SVM) predictors of binding affinity to screen datasets of protein–protein and protein–peptide complexes, as well as to generate and rank mutants of a given structure.
29 de dic. de 2022 · We estimate the binding affinity of human fibroblast growth factor 1 to heparin hexasaccharide based on the available crystal structure of the complex as the initial model and four different...
All samples were loaded on the gel within 20 min from mixing with the loading buffer. Non-denaturing acrylam-ide gels (20%) were pre-run for at least 1 hr at 42 V/cm constant voltage, 4–6 ̊C with 0.5x TBE buffer. (50 mM Tris, 42 mM boric acid, 0.5 mM EDTA.Na2, pH 8.5–8.6 final) using a circulating cooling sys-tem.