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MudPIT is a non-gel technique for separating and identifying individual components of complex protein and peptide mixtures. It uses multidimensional liquid chromatography and mass spectrometry to analyze proteins in various biological samples, such as cancer cells.
Multidimensional Protein Identification Technology (MudPIT) eliminates gel separations. Instead, biochemical fractions containing many proteins are directly proteolyzed and the enormous number of peptides generated, are separated by 2-dimensional liquid chromatography before entering the mass spectrometer.
MudPIT is a method that uses cation exchange and reverse phase HPLC to separate and identify peptides from a proteome sample. It can be applied to whole proteome analysis, SILAC experiments, and dual enzymatic digestion for increased coverage.
1 de ago. de 2005 · The archetypal approach, termed MudPIT (for Multidimensional Protein Identification Technology) [19], pioneered in the laboratory of John Yates, III, has proven to be a remarkably effective and robust methodology for investigating global changes in protein expression as a function of development and disease 20, 21, 22.
1 de ene. de 2009 · One of these technologies is multidimensional protein identification technology (MudPIT). It consists of two orthogonal separation systems – strong cation exchange (SCX) and reversed phase (RP) – coupled online in an automated fashion to mass spectrometric detection.
MUDPIT (multidimensional protein identification technology) is the method of choice for complex protein sample analysis in which more elaborate separation techniques are needed. For MUDPIT analysis strong cation exchange resin (SCX), which binds positively charged compounds, is packed in tandem to the RP-C18 resin.
1 de oct. de 2003 · Multidimensional Protein Identification Technology (MudPIT) eliminates gel separations. Instead, biochemical fractions containing many proteins are directly proteolyzed and the enormous number of peptides generated, are separated by 2-dimensional liquid chromatography before entering the mass spectrometer.
