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Hace 23 horas · Molecular Biology Reports - Yersinia ... (ELASA) and surface plasmon resonance (SPR) were used to determine the affinity and specificity of obtained aptamers to F1 protein. The analysis showed that among the obtained aptamers, the three aptamers of Yer 21, Yer 24, and Yer 25 were selected with a KD value of 1.344E − 7, 2.004E − 8 ...
Hace 5 días · His-tags are the most used and cost-effective affinity tags to purify recombinant proteins using an immobilized metal affinity chromatography (IMAC) column (Porath et al., 1975).Notably, protein activity is rarely affected by polyhistidine affinity tags because of their relatively small size and charge (Block et al., 2009).In the context of H. volcanii strains where naturally histidine-rich ...
Hace 4 días · Higher-affinity germline-targeting immunogens extended ... and the line denotes the median 10E8-UCA H /mouse L value. b, ... Experiments were performed on a ProteOn XPR36 (Bio-Rad) using a GLC ...
Hace 3 días · Blood values. Clinical significance. High or low levels. Type 2 diabetes. Coagulation. Medications. Endogenous steroids. Measurement. Affinity and binding. Synonyms. References. Further reading. External links. Sex hormone-binding globulin ( SHBG) or sex steroid-binding globulin ( SSBG) is a glycoprotein that binds to androgens and estrogens.
Hace 5 días · Affinity-directed substrate/H + antiport by PfMATE. We also found that a substrate, ethidium, exhibits significantly higher binding affinity to the IF conformation, with a Kd of approximately 40 μM ( Figure 8 ), compared to the OF conformation, where the binding affinity exceeds 1 mM ( Figure 7 ).
Hace 23 horas · Through quantitative analysis, five mAbs with high affinity were generated and their half-maximal effective concentration (EC 50) values reached the ≈ 10 –13 M scale. These mAbs have the potential to be utilized for the early identification of PEDV infection, and our research provides a solid foundation for further investigation.
Hace 3 días · Sequencing is used in molecular biology to study genomes and the proteins they encode. Information obtained using sequencing allows researchers to identify changes in genes and noncoding DNA (including regulatory sequences), associations with diseases and phenotypes, and identify potential drug targets. Evolutionary biology
