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31 de dic. de 2019 · The term ‘Ki’ is used whenever this binding constant is measured through inhibition kinetics, while ‘Kd’ is preferred when the binding is measured more directly (e.g. by fluorescence quenching, isothermal titration calorimetry, or surface plasmon resonance). IC50 vs Ki. IC50 stands for inhibitory concentration 50%.
29 de dic. de 2017 · Affinity in chemistry is the tendency of dissimilar chemical species to form chemical compounds. Dissociation constant (Kd ) is the rate constant of dissociation at equilibrium, defined as the ratio koff / kon (where koff and kon are the rate constants of association of the drug off and on to the receptor). When Ka is high, Kd is low, and the drug has a high affinity for the receptor (fewer ...
30 de jul. de 2018 · Introduction. Docking, originally introduced by Kuntz et al. [ 1 ], is a computational method that virtually tries to predict a complex of (usually) two binding partners. Typically, these binding partners are biological macromolecules (e.g., protein, DNA/RNA, peptide) or small molecules (e.g., endogenous ligands, drugs).
The Michaelis-Menten model describes the kinetics of such enzyme catalysed reactions. In this model, two values are used to describe an enzyme catalysed reaction, the maximal rate or maximal velocity ( Vmax) and the Michaelis-Menten constant ( Km) These values are derived from the reaction rate at different substrate concentrations.
The binding affinities of 12 different HS/heparin oligosaccharides (see Fig. 9.5, 1 – 8 and 10 – 13) for FGFs were assayed using the microarray. FGF-2 bound strongly to hexasaccharides 1, 2, and 5, tetrasaccharide 7, and had weak affinity for disaccharide 10 and monosaccharide 12. No binding was observed for hexasaccharides 3, 4, and 6.
29 de nov. de 2021 · The smaller K m value for ARF6 peptide with myristoyl-CoA indicates that NMT1 interacts with ARF6 peptide more efficiently toward catalysis after binding myristoyl-CoA than after ... –substrate pairs can coimmunoprecipitate strongly suggests that the determination of substrate specificity by binding affinity is prevalent in biology.
16 de oct. de 2014 · Slightly lower AUC values are obtained when distinguishing between medium- and low-affinity complexes (Figure 4, green curves). However, worse results are obtained when distinguishing high-affinity complexes from medium-affinity complexes, pointing to apparent similarity of these two groups ( Figure 4 , blue curves).
