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  1. Hace 3 días · Distance Vector routing protocols use frequent broadcasts (255.255.255.255 or FF:FF:FF:FF) of their entire routing table every 30 sec. on all their interfaces in order to communicate with their neighbours. The bigger the routing tables, the more broadcasts. This methodology limits significantly the size of network on which Distance Vector can be used.

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  3. Hace 4 días · A Support Vector Machine (SVM) is a supervised machine learning algorithm used for classification and regression tasks. SVM works by finding a hyperplane in a high-dimensional space that best separates data into different classes. It aims to maximize the margin (the distance between the hyperplane and the nearest data points of each class ...

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  6. Hace 2 días · Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system. Activation of expression is achieved by providing T7 RNA polymerase within the cell.

  7. Hace 4 días · A lentivirus CRISPR vector is first constructed as a plasmid in E. coli. The gRNA and Cas9 expression cassette is cloned between the two long terminal repeats (LTRs) during vector construction. A human U6 promoter drives the expression of the user-selected gRNA sequence, which directs Cas9 to the DNA target site of interest.