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6 de ago. de 2020 · Assessment of published K D values for RNA-binding proteins. We analyzed 100 papers reporting K D or ‘apparent K D ’ values of RNA/protein interactions. Measurements were evaluated based on two criteria: demonstrating equilibration (horizontal axis) and controlling for titration (vertical axis).
6 de ago. de 2020 · Moreover, several reported affinities could be concluded to be incorrect, thereby impacting biological interpretations. Given these challenges, we provide a framework for a broad range of researchers to evaluate, teach about, perform, and clearly document high-quality equilibrium binding measurements.
View our guide to KD value. Includes how KD correlates to antibody affinity and sensitivity, how KD values are measured and read and KD value FAQs.
16 de oct. de 2014 · Abstract. Protein-protein interactions (PPI) in nature are conveyed by a multitude of binding modes involving various surfaces, secondary structure elements and intermolecular interactions. This diversity results in PPI binding affinities that span more than nine orders of magnitude.
11 de nov. de 2020 · This study demonstrates that machine learning is a powerful framework for combining different approaches to predict antibody affinity changes.
29 de nov. de 2021 · This observation indicates that binding affinity is a more effective predictor of substrate specificity than k cat /K m. Our findings have important biological and medicinal implications warranting additional considerations for enzyme substrate and inhibitor discovery.
31 de mar. de 2023 · Drug-target binding affinity prediction plays a key role in the early stage of drug discovery. Numerous experimental and data-driven approaches have been developed for predicting drug-target binding affinity.
