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16 de may. de 2024 · A multiplex PCR (or mPCR) approach was designed to assess possible plasmid transfer events from the donor strain to a recipient and incorporated seven sets of primers, each targeting unique sequences within each of the six plasmids (and a chromosomal control) from L. lactis UC11.
18 de may. de 2024 · open access. Highlights. •. Endophytic bacteria from Solanum hindsianum exhibit plant growth-promoting and pathogen suppression traits. •. Selected Pseudomonas strains for colonization assays tagged with Tn7 transposon carrying kanamycin resistance and sGFP2 gene. •.
2 de may. de 2024 · Colony PCR (Kong et al., 2005) was performed from a total of 159 colonies grown from three-day PDA cultures, using Cps- and Che-specific primers (Guo & Pooler, 2021). Also included in the PCR were three French isolates from the ANSES collection: LSVM0960 collected by Department Île-de-France in 2013, LSVM1007 by Department Meurthe ...
Hace 2 días · Single-colony PCR was performed to identify the different variant types of S. communis B10998 in the population Full size image Finally, to confirm that the mutation in mglB influenced the gut colonization characteristics of B10998, we assayed for competitive advantage by competing the mutant SA01065 against the background strain SA01065’ and the WT in both honey and bumble bee hosts.
Hace 1 día · Genes were cloned using restriction ligation or by Gibson assembly and confirmed via colony PCR, digestion, and sequencing. The plasmids pSP-GM1 (accession number: Addgene #64739) (44) and pBEVY_GL (accession number: Addgene #51225) (45) were used for the construction of the integration cassettes, placing the genes under the control of the respective promoters.
Hace 2 días · Positive clones were screened using colony PCR and sequenced by a sequencing company for verification. The correctly sequenced recombinant vector was named pET28a-NecR. The P neoS and transcription factor NecR gene fragments were amplified using pri-mers PneoS-eGFP F/PneoS-eGFP R and NecR-PneoS-eGFP F/NecR-PneoS-eGFP R, ...
1 de may. de 2024 · KAPA2G Fast HotStart ReadyMix with dye (2X) (Nippon Genetics Co., Ltd., Tokyo, Japan) or EmeraldAmp MAX PCR Master Mix (2 × Premix) (Takara Bio) was used for colony PCR.
