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Hace 5 horas · Real-time polymerase chain reaction quantification. Total RNA was extracted from HepaRG cells using TRI reagent (Sigma-Aldrich) according to the manufacturer’s instructions, and the total RNA concentration was quantified using a spectrophotometer (A260/A280).
Hace 5 horas · A new study encompassing nearly 40,000 hospital visits from a geographically diverse healthcare database shows that sampling a single stool, using multiple polymerase chain reaction (PCR) panels, can identify more pathogens, notably diarrhea-causing E. coli and enteric viruses, and do so more rapidly than a conventional workup.
Hace 5 horas · Molecular Methods: Techniques like PCR (Polymerase Chain Reaction) for rapid detection and identification. Common Microbes That Affect Fruits. Bacteria: Salmonella spp.: Often found in contaminated water used for irrigation. It can cause severe foodborne illnesses.
Hace 5 horas · Molecular diagnostics techniques are methods used to analyze biological markers in the genome and proteome. These techniques are used to detect and measure the presence of genetic material or proteins associated with a specific health condition or disease. The techniques include: Step 2/9. 1. Polymerase Chain Reaction (PCR): This is the most ...
Hace 5 horas · Following this, the minimal quantity of DNA collected undergoes PCR (polymerase chain reaction), which allows scientists to replicate/amplify the DNA. PCR involves denaturing the DNA, then elongating it, and finally copying it. Denaturation is done by exposing DNA to a specific temperature, and replication is done by adding a specific DNA ...
Hace 5 horas · Expression of VEGF-A and PDGF-A, PDGF-B, PDGF-C, PDGF-D genes was measured after total RNA extraction and cDNA synthesis, using real-time polymerase chain reaction (RT-PCR). RESULTS: The highest entrapment efficiency (EE) was achieved by Span 60:cholesterol (1:1) with 64.3%.
Hace 5 horas · SYBR Premix Ex Tap (Vazyme Biotechnology Co., Ltd., Nanjing, China) was used for real-time quantitative polymerase chain reaction (qPCR) on an ABI PRISM 7500 (Applied Biosystems, Foster City, CA, USA). Table S1 (additional file 1) shows the primer sequences used for qPCR.
