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1 de mar. de 2009 · Myers and Varela-Diaz using the media described by Fletcher (1928) and Ellinghausen and McCullough (1965) with the addition of 200 μg/mL 5-fluorouracil, indicated the need to subculture later into an antibiotic containing medium, for ... The Ellinghausen, McCullough, Johnson and Harris - EMJH ...
Leptospira are cultivated at 30 °C in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, which can be supplemented with 0.21% rabbit serum to enhance growth of fastidious strains. Growth of pathogenic Leptospira in an artificial nutrient environment such as EMJH becomes noticeable in 4–7 days; growth of saprophytic strains occur within 2–3 days.
8 de jul. de 2020 · Ellinghausen HC Jr, McCullough WG (1965) ... Johnson RC, Harris VG (1967) Differentiation of pathogenic and saprophytic Leptospires. I. Growth at low temperatures. J Bacteriol 94(1):27–31. Article CAS Google Scholar Johnson RC, Rogers P (1964) 5-Fluorouracil as a selective agent for growth of Leptospirae. J Bacteriol 87:422 ...
26 de oct. de 2022 · Among multiple culture media evaluated, Ellinghausen-McCullough-Johnson-Harris (EMJH) media containing antimicrobial agents was superior in recovering and detecting Leptospira from the environmental samples. Our results show that enrichment culture followed by PCR can be used to confirm the presence of pathogenic Leptospira in environmental ...
EMJH (Ellinghausen-McCullough-Johnson-Harris) medium was prepared by adding 100 ml of Leptospira Enrichment EMJH to 900 ml Leptospira Medium Base EMJH (BD Difco, Sparks, MD, USA), supplemented with 5-Fluoruracil (5-FU, 100ug/ml, Sigma F-6627).
1 de mar. de 2013 · The common maintenance method entails a periodic sub-culture into fresh media either in liquid or semisolid Ellinghausen and McCullough modified by Johnson and Harris (EMJH) and Fletcher media at ...
As amostras de urina obtidas de cada animal foram imediatamente semeadas em tubos contendo meio de cultura Ellinghausen-McCullough-Johnson-Harris (EMJH). Das 15 amostras de urina avaliadas, duas leptospiras foram isoladas e nomeadas Londrina 49 e Londrina 54.
