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EMJH (Ellinghausen-McCullough-Johnson-Harris) medium was prepared by adding 100 ml of Leptospira Enrichment EMJH to 900 ml Leptospira Medium Base EMJH (BD Difco, Sparks, MD, USA), supplemented with 5-Fluoruracil (5-FU, 100ug/ml, Sigma F-6627).
8 de jul. de 2020 · Ellinghausen HC Jr, McCullough WG (1965) ... Johnson RC, Harris VG (1967) Differentiation of pathogenic and saprophytic Leptospires. I. Growth at low temperatures. J Bacteriol 94(1):27–31. Article CAS Google Scholar Johnson RC, Rogers P (1964) 5-Fluorouracil as a selective agent for growth of Leptospirae. J Bacteriol 87:422 ...
Resumen Realizamos cambios en la formulación original del enriquecimiento del medio Ellinghausen-McCullough-Johnson-Harris y evaluamos algunos aspectos, como el tiempo de crecimiento de Leptospira y la utilización en la prueba de aglutinación microscópica (MAT), comparándolos con el enriquecimiento original y un medio de enriquecimiento comercialmente disponible (DIFCOÔ).
dios comunmente usados son el Ellinghausen-McCullough-Johnson-Harris medium (EMJH) el cual contiene 1% BSA y Tween 80; Korthoff’s y Fletcher’s5. Rev Peru Med Exp Salud Publica 22(4), 2005 292 Céspedes M. EPIDEMIOLOGÍA La leptospirosis es una zoonosis de distribución mun-
Among multiple culture media evaluated, Ellinghausen-McCullough-Johnson-Harris (EMJH) media containing antimicrobial agents was superior in recovering and detecting Leptospira from the environmental samples. Our results show that enrichment culture followed by PCR can be used to confirm the presence of pathogenic Leptospira in environmental ...
The viability of six serovars of Leptospira spp. was studied after long storage at -70°C. The bacteria were either preserved in Ellinghausen-McCullough-Johnson-Harris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark-ground microscopy over a period of ...
Ellinghausen-McCullough-Johnson-Harris is listed in the World's most authoritative dictionary of abbreviations and acronyms. Ellinghausen-McCullough-Johnson-Harris - What does Ellinghausen-McCullough-Johnson-Harris stand for? The Free Dictionary.
